Keywords:Antioxidant, Xanthophylls, anthocyanins, lignans, stilbenes, Gamma Oryzenol

ABSTRACT

The most successful sources of therapeutically active medicines have been natural compounds referred to as secondary metabolites. Nevertheless, there has been a noticeable drop in interest in their usage in medication research and discovery recently. On the other hand, compared to traditional combinatorial chemistry, which primarily offers potential for the identification of new low molecular weight molecules, plant natural products offer structural variety. The difficulty lies in gaining access to this natural chemical variety, as less than 10% of the world's biodiversity has had its potential biological activity evaluated. Many valuable natural lead compounds are still undiscovered. Compared to vitamin E, gamma-oryzanol has a stronger antioxidant action that lowers cholesterol oxidation. To determine whether gamma-oryzanol may be used to treat hepatocellular cancer, the results of the MTT test were examined. One of the main characteristics of this kind of cancer has been shown to be an increase in oxidative stress due to an imbalance in the oxidant and antioxidant system. The purpose of this study was to assess gamma-oryzanol's antioxidant capacity in a hepatocellular cancer cell line (HepG-2). After a 7-day treatment, the effect of gamma-oritannol on HepG-2 was assessed using the MTT reagent proliferation assay with IC50. In the test of in vitro proliferation conditions (MTT), the IC50 values are high (mg/ml). To summarise, the findings indicate that gamma-oryzanol has the potential to impact hepatocellular carcinoma. Additionally, it may play a significant role in adjuvant cancer therapy by targeting cancer cells for more effective cytotoxic drugs, as well as aiding in cancer chemoprotection.